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. 2021 Jan 20;13(2):147. doi: 10.3390/v13020147

Figure 1.

Figure 1

Heparin inhibition by of ALFV3929 and other small plaque ALFV derivatives. Viruses were incubated with heparin (50 µg/mL or 200 µg/mL) for 15 min prior to their addition to SW13 (A,C) or C6/36 (B,D) cell monolayers. Error bars indicate the standard deviation values calculated for each triplicate. DM stands for the double mutant which contains the MVEV sequence in both the hinge and glycosylation sites. Percentage inhibition of titre caused by heparin was calculated as follows: 100 − (TCID50 in the presence of heparin/TCID50 of mock treated control) × 100%. MVE/ALFVstr-K327Q-DM = MVE/ALFstr-CHO+/HMVEV/K327Q, MVE/ALFstr-K327Q-Hinge = MVE/ALFstr-HMVEV/K327Q.