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. 2021 Jan 20;13(2):73. doi: 10.3390/toxins13020073

Figure 5.

Figure 5

Effect of DON on apoptosis-related protein expression: (A) Western blot was used to detect proteins expression; (B) The effect of DON on Caspase3, Caspase9, cleaved-Caspase9(cle-Caspase9), cleaved-Caspase3(cle-Caspase3), Cyt C and AIF expression. The protein expression of Mito Cyt C, Mito AIF, Caspase 3, and Caspase 9 were decreased, and the protein expression of Cyto Cyt C, Cyto AIF, cle-Caspase3 and cle-Caspase9 were dose-dependent increased with the increase of DON concentration, these effects were greatest when DON concentration was 1000 ng/mL. Caspase-8 inhibitor ZIF increased the expression of Mito Cyt C, Mito AIF, Caspase 3, and Caspase 9 and decreased the expression of Cyto Cyt C, Cyto AIF, cle-Caspase3, and cle-Caspase9. Data are mean values ± SD (n = 3). * Significant difference vs. controls (p < 0.05). ** Highly significant difference vs. controls (p < 0.01). ## Highly significant difference vs. 1000 ng/mL DON group (p < 0.01).