Table 1.
Central composite design and results of dependent variables in PLE from pomegranate peel.
| Run | X1 | X2 | Temperature [X1] | Ethanol [X2] | TPC | Punicalagin | AMA |
|---|---|---|---|---|---|---|---|
| (°C) | (%) | (mg GAE/g DW) | (mg/g DW) | S. aureus (mm) | |||
| 1 | −1 | −1 | 55 | 10 | 14.1 ± 0.7 | 7.7 ± 0.2 | 2.0 ± 0.4 |
| 2 | −1 | 1 | 55 | 90 | 75.9 ± 3.0 | 38.0 ± 1.9 | 7.5 ± 0.7 |
| 3 | 1 | −1 | 185 | 10 | 73.0 ± 1.0 | 5.0 ± 0.2 | 4.0 ± 0.7 |
| 4 | 1 | 1 | 185 | 90 | 129.6 ± 6.3 | 30.1 ± 0.9 | 7.0 ± 2.8 |
| 5 | −1.21 | 0 | 41.3 | 50 | 39.6 ± 1.6 | 36.1 ± 0.6 | 6.0 ± 0.0 |
| 6 | +1.21 | 0 | 198.6 | 50 | 149.0 ± 5.3 | 22.0 ± 0.3 | 8.3 ± 1.1 |
| 7 | 0 | −1.21 | 120 | 1.6 | 20.0 ± 3.5 | 4.0 ± 0.3 | 3.0 ± 0.7 |
| 8 | 0 | +1.21 | 120 | 98.4 | 40.9 ± 1.9 | 23.3 ± 1.3 | 5.5 ± 1.4 |
| 9 | 0 | 0 | 120 | 50 | 39.2 ± 0.2 | 38.6 ± 2.5 | 4.8 ± 1.1 |
| 10 | 0 | 0 | 120 | 50 | 27.7 ± 0.5 | 35.0 ± 1.5 | 3.0 ± 0.7 |
| 11 | 0 | 0 | 120 | 50 | 33.2 ± 1.0 | 31.4 ± 0.6 | 3.8 ± 0.4 |
| 12 | 0 | 0 | 120 | 50 | 39.0 ± 0.2 | 38.0 ± 1.1 | 4.8 ± 0.4 |
Data represent mean ± standard deviation. TPC, total phenolic content; AMA, antimicrobial activity.