Figure 3. Anti-oxidants Attenuate ROS Accumulation and Premalignant Cholangiocellular Lesions in Hspd1^ΔLPC Mice.

(A) Survival plots for Hspd1^ΔLPC mice on normal or BHA diet.

(B) Macroscopy of the liver of 6- and 8-week-old Hspd1^ΔLPC or 8-week-old WT mice on normal or BHA diet. Scale bar, 1 cm.

(C) H&E of the liver of 8-week-old Hspd1^ΔLPC mice on normal versus BHA diet and quantification of necrotic areas. Scale bar, 100 μm.

(D) Serum ALT, AST, and bilirubin in 8-week-old Hspd1^ΔLPC mice on normal and BHA diet.

(E) A6 IHC in 8-week-old normal versus BHA diet Hspd1^ΔLPC livers and quantification of cholangiocellular overgrowth. Scale bar, 20 μm.

(F) qRT-PCR of livers from 8- and 20-week-old normal versus BHA diet Hspd1^ΔLPC mice for CK19 and Hnf4α.

(G and H) Western blot of liver lysates from 8-week-old normal versus BHA diet Hspd1^ΔLPC mice (G) and quantifications the p-JNK to total JNK and the p-Erk1/2 to total Erk1/2 ratio (H).

(I) IHC for p-JNK and CHOP in consecutive liver sections from normal versus BHA diet 8-week-old Hspd1^ΔLPC mice. Scale bar, 50 μm.

(J) qRT-PCR of livers from 8- and 20-week-old normal versus BHA diet Hspd1^ΔLPC mice for genes indicated.

(K) A schematic depicting the action of BHA in Hspd1^ΔLPC mice.

Data are represented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant. See also Figure S4.