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. 2021 Feb;92:157–164. doi: 10.1016/j.bbi.2020.12.003

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig 4 — Macrophages and microglia production during reference memory task. Single-cell suspensions from the brain stem, thalamus and hippocampus were examined by FACS for CD45 + CD11b + macrophages and CD45-CD11b + microglia following the task of reference memory in MWM for wild-type and IL-4Rα^-/- mice. (a) A gating strategy for CD45 + CD11b + and CD45-CD11b + cell population is shown. To determine macrophage and microglia populations from CD11b + cells, CD45 + CD11b + and CD45-CD11b + cells were gated on, showing significantly more CD45-CD11b + cells by wild-type and IL-4Rα^-/- mice, compared to CD45 + CD11b + in the (b) brainstem, (c) thalamus, and (d) hippocampus. Data shown represents means ± SEM; n = 6 mice per group. * P < 0.05; **P < 0.01; ***P < 0.001; **** P < 0.0001.