Figure 4.

Effect of temperature on EV production. (A) S. aureus was cultivated in 100 mL tryptic soy both (TSB) at the indicated temperatures until an OD of 1 was achieved. (B) EV production quantified by relative protein yield (compared to 37 °C cultures) or (C) by reactivity of dot immunoblots of EV suspensions with indicated antibodies. Dot bots were performed at least three times, and a representative image is presented. (D) Mean relative intensity values (compared to 37 °C cultures) pooled from 3 independent immunoblot experiments are shown. One-way ANOVA with Dunnett’s multiple comparison tests were used for statistical analyses of protein yield and relative intensity of dot blot images. * p < 0.05, **** p < 0.0001.