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. 2021 Jan 20;13(2):75. doi: 10.3390/toxins13020075

Figure 7.

Figure 7

S. aureus EV production was reduced by osmotic stress. (A) EV production from S. aureus grown in TSB supplemented with 1 to 2% NaCl was evaluated by quantification of relative EV protein yield or (B) by dot immunoblots of EV suspensions probed with indicated antibodies. (C) Relative intensity of dot blot images pooled from three independent experiments are shown, and a representative blot is shown. EV protein yield was analyzed from three independent experiments. One-way ANOVA with Dunnett’s multiple comparison tests were used for statistical analysis of protein yield and relative intensity of dot blot images. *p < 0.05, ** p < 0.01.