Figure 3. Gating strategy to sort out uninfected, productively and latently infected cells.

A. Set the gate on live cells. Cell viability is monitored by forward (FSC-Area) and side scatter (SSC-Area) analysis. B and C. Gate successively on singlets FSC-Area vs. FSC-Width, and SSC-Area vs. SSC-Width. D. Set the gate on GFP/FITC-Area⁺ to sort productively infected cells, or on GFP/FITC-Area- vs. mKO2/PE-Area⁺ to sort latently infected cells, or to GFP/FITC-Area- vs. mKO2/PE-Area- to sort uninfected cells. Run briefly each sorted sample when the sort is over to check purity (usually > 90%).