FIGURE 1.

Fat accumulation is decreased with low‐dose but not with high‐dose α‐tocopherol supplementation (A) Representative hematoxylin and eosin staining in the liver derived from each group (B) Quantitation of triglyceride contents in the liver of each group (n = 10 in each) (C) Serum α‐tocopherol levels (D) Serum potential antioxidant (PAO) assay. *p < 0.05 versus Standard, **p < 0.01 versus Standard, ^† p < 0.05 versus high‐fat (HF), ^†† p < 0.01 versus HF, ^‡ p < 0.05 versus HF + low α‐Toc, ^‡‡ p < 0.01 versus HF + low α‐Toc. Standard, standard including vitamin E acetate‐fed; HF, HF including vitamin E acetate‐fed; HF + low α‐Toc, HF‐fed with α‐tocopherol (50 mg/kg) supplementation; HF + high α‐Toc, HF‐fed with α‐tocopherol (200 mg/kg) supplementation. Data are shown as mean ± SD. Scale bar = 20 μm