FIGURE 2.

carnitine palmitoyltransferase I (CPT‐1) protein levels in the liver are elevated with low‐dose but not with high‐dose α‐Toc supplementation(A) Western blotting and densitometry analysis were performed to determine hepatic protein levels of mouse CPT‐1, peroxisome proliferator‐activated receptor alpha (PPARα), uncoupling protein 2 (UCP‐2), acetyl‐CoA carboxylase α (ACACA), sterol regulatory element‐binding protein 1 (SREBP‐1), fatty acid synthase (FAS), and β‐actin in each group. (B–G) The relative quantification of CPT‐1 (B), PPARα (C), UCP‐2 (D), ACACA (E), and FAS (G) band intensity divided by β‐actin intensity. Data are shown as mean ± SD. ^* p < 0.05 versus Standard, ^†† p < 0.01 versus HF, ^‡‡ p < 0.01. Standard, standard including vitamin E acetate‐fed; high‐fat (HF), HF including vitamin E acetate‐fed; HF + low α‐Toc, HF‐fed with α‐tocopherol (50 mg/kg) supplementation; HF + high α‐Toc, HF‐fed with α‐tocopherol (200 mg/kg) supplementation