Table 1. Summary of data on T. brucei nucleotide sugar biosynthetic enzymes.

Data are from the literature [#] or from this paper [TP]. GL = glycosomal; CP = cytoplasmic; IFM = immunofluorescence microscopy; *indicates that IFM was performed using an epitope tag rather than an antibody to the whole protein; PTS = peroxisome targeting sequence (type 1 or type 2) for the respective T. brucei (Tb), T. cruzi (Tc) and L. major (Lm) enzymes [50,65]. Enzyme abbreviations are listed in the Introduction, with the exception of TbGFAT, glutamine: fructose-6-phosphate aminotransferase.

Enzyme	Gene	Essential for cell survival		Protein expression, crystallography and antibodies			IFM		Digitonin Latency		Sub-cellular Fraction ation		Glycosome proteome		PTS
		bsf	pcf	Prot	Xtal	Ab	bsf	pcf	bsf	pcf	bsf	pcf	bsf	pcf	Tb	Tc	Lm
TbHK1	Tb927.10.2010	[62] [63]		[60]		[59]	GL [59]	GL [59]	GL [58] [59]	GL [57] [59]	GL [55] [56]	GL [57] [58]	GL [53]	GL [54] [TP]	2	2	2
TbPGI	Tb927.1.3830			[64]	[64]				GL [59]		GL [55] [56]	GL [57]		GL [53] [54] [TP]	1	1	1
TbUGP	Tb927.10.13130	[45]		[45]	[45]	[45]	GL [45]	GL [TP]	GL [TP]	GL [TP]		GL [TP]		GL [54]			1
TbGALE	Tb927.11.2730	[41]	[44]	[41]	[43]	[44] [TP]	GL [44]	GL [44] [TP]	GL [TP]	GL [TP]	GL [44]			GL [54]	1		2
TbGFAT	Tb927.7.5560
TbGNA	Tb927.11.11100	[49]		[49]	[49]	[49]	GL [49]	GL [TP]
TbPAGM	Tb927.8.980	[42]		[42]		[42]	GL [42]	GL+CP [TP]	GL+ CP [42]	GL+ CP [TP]
TbUAP	Tb927.11.2520	[50]		[50]	[50]	[50] [TP]	GL [50]	GL [TP]	GL [TP]	GL [TP]				GL [54]	1	1	1
TbPMI	Tb927.11.14780	[46]		[46]		[46]	GL [46]	GL [TP]	GL [TP]	GL [TP]		GL [TP]		GL [53] [54] [TP]	1	1
TbPMM	Tb927.10.6440	[42]		[42]	[42]	[42]	GL [42]	GL [TP]	GL [42] [TP]	GL [TP]						1	1
TbMPGT	Tb927.8.2050	[47]		[47] [48] [TP]		[TP]	CP [48]	GL [TP]	GL [TP]	GL [TP]		GL [TP]
TbGMD	Tb927.10.15490	[52]	[52]	[52]				GL [52]*
TbGMER	Tb927.11.13990			[52]		[TP]		GL [TP]	GL [TP]	GL [TP]