Table 3. Quantitative analysis of anti-NS enzyme and anti-TbGAPDH colocalization by IFM in pcf cells.
NS Enzyme | na | Mander’s colocalization coefficientb |
---|---|---|
TbGNA | 6 | 0.85 ± 0.05 |
TbPAGM | 5 | 0.33 ± 0.06 |
TbUAP | 5 | 0.89 ± 0.02 |
TbPMI | 9 | 0.96 ± 0.01 |
TbPMM | 4 | 0.73 ± 0.06 |
TbMPGT | 4 | 0.89 ± 0.04 |
TbUGP | 8 | 0.89 ± 0.03 |
TbGalE | 4 | 0.88 ± 0.05 |
TbGMER | 7 | 0.88 ± 0.05 |
a n is number of cells uses in the analysis.
b The Mander’s colocalization coefficient (± standard deviation of the mean) is a measure of the amount of green (anti-NS enzyme) signal above background that colocalizes with red (anti-TbGAPDH) stained compartments.