Fig 7. Dynein preassembly mutant sperm flagella and respiratory cilia show absence of the IDA intermediate light chain DNALI1.

Control (A) and mutant (B-F) sperm, as well as control (G) and mutant (H-L) respiratory cells were double-labeled with antibodies directed against acetylated α tubulin (green) and the inner dynein arm intermediate light chain DNALI1 (red). Both antibodies co-localize along the flagella and cilia in cells from the unaffected control (yellow, A, G). In all mutant sperm flagella (B-F) and respiratory cells (H-L) DNALI1 is absent or severely reduced from the flagellar and ciliary axonemes. Nuclei are stained with Hoechst33342 (blue). Scale bars represent 10 μm.