Table 2. Primers and optimized PCR conditions used for transcriptional analysis.
| Primer | sequence 5’-> 3’ | quantity unit [a] | annealing (°C) | annealing (s) | [MgCl2] (mM) | [primer] (μM) |
|---|---|---|---|---|---|---|
| RpsL_F1 | TCGTACATCGGCGGTGAAG | 15U | 54 | 7 | 5 | 0,4 |
| RpsL_R1 | CCGCGAACCATGTGGTAAC | |||||
| RpoD_F2 | TGCTGCAGGAAGGCAACCTCG | 15U | 55 | 5 | 4 | 0,4 |
| RpoD_R2 | AAATCGCCTGACGAATCCACC | |||||
| RimM_F2 | CGACAACGGCGTGCATTCGATC | 15U | 50 | 7 | 4 | 0,4 |
| RimM_R2 | GCCTTCACGTACACGCCGACGAAC | |||||
| DnaK_F2 | CGAAATCAACCTGCCGTACATC | 15U | 50 | 7 | 5 | 0,4 |
| DnaK_R2 | CGGGTGATCTTCAGATTCAAGTG | |||||
| RumA_F2 | CATCGTCGCGGTCGGCCACA | 15U | 58 | 5 | 4 | 0,4 |
| RumA_R2 | AGCGCAGTTCGGGCTTCACTTC | |||||
| AmrB_F1 | TCGATCAACGTGCTGACGATG | 15U | 53 | 9 | 5 | 0,6 |
| AmrB_R1 | GCAGCTTCTCCTCGACCATCAG | |||||
| BpeB_F1 | CTCGTCGCGTTGATTCTGAC | 15U | 53 | 9 | 5 | 0,4 |
| BpeB_R1 | AGTTGAAGGTGCGGTTGAAC | |||||
| BpeF_F2 | GGCTTCAACAAGGTGTTCCATC | 30U | 58 | 5 | 4 | 0,5 |
| BpeF_R2 | GGAGATACAGGCCGAGCATCACG |
[a]: 30 U: 1μL of cDNA in 4 μL water; 15 U: 0.5 μL of cDNA in 4.5 μL water.