. Author manuscript; available in PMC: 2021 Apr 1.

Published in final edited form as: Nat Genet. 2020 Mar 23;52(4):363–369. doi: 10.1038/s41588-020-0596-3

Table 1:

A brief list of CRISPR-based technologies with potential applicability to hiPSC-based experiments. More plasmids available at: https://www.addgene.org/crispr/.

	CRISPR Technology	Mammalian Cell Applications	hiPSC/hESC Applications	Addgene No(s).
Genome Engineering	Cas9	HEK293T, HUES9⁷⁵	hiPSCs⁷⁶	62988
	Cpf1	HEK293T⁴⁴	hiPSC⁷⁷	69982
	CasX	HEK293T⁴⁶
Transcriptional Regulation	dCas9-VPR (Gene Activation)	HEK293T⁵¹	hiPSCs⁵¹ hiPSC-derived NPCs, neurons and astrocytes⁷⁸	63798 99373
	dCas9-KRAB (Gene Repression)	HEK293T⁵⁰	hiPSC-derived NPCs, neurons and astrocytes⁷⁸	71237 99372
	dCas9-SunTag (Gene Activation)	U20S⁷⁹	hiPSCs⁸⁰	60904
	SAM (Gene Activation)	HEK293T⁸¹		75112
	dCpf1-VPR (Gene Activation)	HEK293T⁸²		104567
Epigenetic Regulation	dCas9-p300 (Histone Acetylation)	HEK293T⁸³		83889
	dCas9-LSD1 (Histone Demethylation)	Chicken Embryos⁸⁴		92362
	dCas9-DNMT3A or dCas9-MQ1 (Cytosine methylation)	Mouse Embryonic Stem Cells⁸⁵	hESCs⁸⁶	84569
	dCas9-Tet1 (Cytosine demethylation)	Mouse Embryonic Stem Cells⁸⁵	hiPSC-derived neurons⁸⁷	84475
Transcriptome Engineering	Cas13a (RNA Targeting)	HEK293T⁸⁸		91902
	Cas13b (RNA Editing)	HEK293T⁸⁹		103854
	CasRx (Cas13d)	HEK293T⁵⁴	hiPSCs⁵⁴	109049