Table 2:

Kinetic Parameters of the reductive half reaction^a

Enzyme Variant	kred	KD	Kred/KD
XDH WT	127.5 ±8.6	31.4 ±8.3	4.0
XDH Q102G	112.3 ±4.1	54.5 ±6.7	2.1
XDH Q102A	107.9 ±3.3	38.8 ±3.8	2.8
XDH Q197A	130.4 ±6.7	38.2 ±8.4	3.4
XDH Q197E	48.4 ±1.5	28.4 ±4.9	1.7

^aKinetic data were recorded in 50 mM Tris, 1 mM EDTA, pH 8.0.