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. Author manuscript; available in PMC: 2021 Feb 26.
Published in final edited form as: Biochemistry. 2019 Jun 4;58(24):2730–2739. doi: 10.1021/acs.biochem.9b00096

Table 1.

Relative Intensities of Spectral Features from 2D IR Slices in Figure 4a

WT Ca2+ NW1 Ca2+ DDAA Ca2+ NW1-DDAA Ca2+ WT apo
Sbi Smono Sbi Smono Sbi Smono Sbi Smono Sbi Smono
0.47 0.62 0.37 0.50 0.16 0.52 <0.01 0.55 <0.01 0.60
a

Smono, amplitude of the monodentate carboxylate peak; Sbi, amplitude of the bidentate carboxylate peak. All amplitudes are reported as a fraction of the amide I peak amplitude for each sample.