Skip to main content
. 2021 Jan 21;11(2):63. doi: 10.3390/metabo11020063

Figure 1.

Figure 1

Temporal collection and Pulsed-Field Gel Electrophoresis (PFGE) genotyping of clinical P.a isolates responsible for chronic lung infection in CF patients. (a) Representative example of PFGE gels (pulsotypes) of P.a clinical isolates sampled from patients 60 and 141 (1, 2 and 3 represent the sampling time of each isolate: beginning, middle or end of the 2010–2015 follow-up period, respectively; M = marker fragment size). (b) Pairwise comparison of 122 P.a PFGE pulsotypes. Clonal complexes (CCs) and clones have been defined according to Römling et al. criteria [31]: no clonal link if the pulsotypes showed more than 6 different bands, Clonal Complex (CC) if the pulsotypes showed less than 6 different bands, and clonal if the pulsotypes were identical. (c) Temporal series of PFGE-genotyped isolates sampling from patients’ expectorations between 2010 and 2015. Small grey crosses represent all the P.a. isolation time points between 2010 and 2015 for each patient. The symbol shape of the bigger dots represents the CC of the isolates that have been analyzed by PFGE (unique clones are represented by a black cross). For 32/34 patients, the earliest and latest sampled isolates belonging to a same CC have been selected for the Final Cell Bank.