Figure 5.

Mass spectra of reduced and S-glutathionylated PRX-IIE. S-glutathionylation was carried out by disulfide exchange with GSSG. The reaction mixtures containing 30 µM reduced PRX-IIE without (left) or with 10 mM GSSG (right) in Tris buffer, pH 8.0, were incubated at 4 °C for 18 h. The protein products were precipitated and subsequently subjected to ESI-MS analysis.