Figure 6.

S-glutathionylation of PRX-IIE. (A) Deconvoluted mass spectra of reduced and S-glutathionylated PRX-IIE after treatment with oxidized glutathione (GSSG). Reduced PRX-IIE (19,438 Da) was treated with indicated GSSG concentrations and the degree of modification was estimated from the mass shift of 306 Da to 19,744 Da, which could be assigned to monoglutathionylated protein. Data are representative spectra of n = 12, with protein from three independent protein purifications. (B) Western blot analyses of reduced and S-glutathionylated PRX-IIE (treatment with 10 mM GSSG) using specific anti-GSH and anti-PRX-IIE antibody. (C) Time dependence of S-glutathionylation with recombinant PRX-IIE in vitro.