Figure 9.

Detection of glutathionylated PRX-IIE species in vivo. A. thaliana plants were stressed for 3 h with 300 µM MV (blot on the right-hand side) or 0.1% (v/v) Tween-20 as control (blot on the left-hand side). S-glutathionylation was detected with specific anti-PRX-IIE antibody following separation by non-reducing 2D-SDS-PAGE and blotting. The spots of the two blots were cropped and zoomed in to clearly show the shift of the spots induced by MV treatment (the bottom part of the figure) The figure shows representative Western blots from two independent experiments.