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. 2021 Feb 26;7(9):eabd3666. doi: 10.1126/sciadv.abd3666

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 4 — (A) Schematic of in vivo fiber photometry and behavior. (B) Average z-scored calcium transient responses of BNST^vGAT-PBN terminals to an aversive shock (n = 7 mice). (C) Mean z-scored calcium transient responses of BNST^vGAT-PBN terminals (n = 7 mice) 10 s before and after the shock initiation. (D) Representative heatmap of BNST^vGAT-PBN terminal calcium transient activity of a single mouse during aversive shock presentation. (E) Average z-scored calcium transient responses of BNST^vGLUT2-PBN terminals to an aversive shock (n = 6 mice). (F) Mean z-scored calcium transient responses of BNST^vGLUT2-PBN terminals (n = 6 mice) 10 s before and after the shock initiation. (G) Representative heatmap of BNST^vGLUT2-PBN terminal calcium transient activity of a single mouse during aversive shock presentation. (H) Average z-scored calcium transient responses of BNST^vGAT-PBN terminals to entry into the closed arm of an EZM (n = 6 mice). (I) Mean z-scored calcium transient responses of BNST^vGAT-PBN terminals (n = 6 mice) 5 s before and after entry into the closed arm of an EZM. (J) Mean z-scored calcium transient responses of BNST^vGAT-PBN terminals (n = 6 mice) 5 s before and after entry into the open arm of an EZM. (K) Average z-scored calcium transient responses of BNST^vGLUT2-PBN terminals to entry into the closed arm of an EZM (n = 5 mice). (L) Mean z-scored calcium transient responses of BNST^vGLUT2-PBN terminals (n = 5 mice) 5 s before and after entry into the closed arm of an EZM. (M) Mean z-scored calcium transient responses of BNST^vGLUT2-PBN terminals (n = 5 mice) 5 s before and after entry into the open arm of an EZM. *P < 0.05, and **P < 0.01. Error bars indicate SEM. See also fig. S3.