Figure 3.

miR-335 targets the 3′-UTR of FTH1 specifically. (A) TargetScan and miRBase were used to investigate the binding site of miR-335 and the 3′-UTR of FTH1. The construction profile of the pLUC-FTH1 plasmid is presented. The binding sequence of 3′UTR of FTH1 was replaced by the pLUC-FTH1 MUT plasmid. (B) Luciferase activity of miR-335 inhibitor (100 nM), miR-335 mimic (100 nM), miR-335 inhibitor negative control (100 nM) or miR-335 mimic negative control (100 nM) in the presence of the FTH1 WT (200 ng) or FTH1 MUT (200 ng) was detected using the dual luciferase reporter assay. ^*P<0.05 vs. miR-335 inhibitor NC + FTH1 WT group; ^**P<0.01 vs. miR-335 mimic NC + FTH1 WT group. FTH1, ferritin heavy chain 1; WT, wild-type; MUT, mutated type.