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Effect of NPPB on apoptosis in HLECs. HLECs were incubated with 50 µM NPPB for 24 h, and the expression levels of the apoptosis markers BAX, Bcl-2 and cleaved caspase-3 were determined by western blotting. β-actin was used as an internal control. The results are presented as the mean ± standard deviation (n=3). *P<0.05. HLEC, human lens epithelial cell; NPPB, 5-nitro-2-(3-phenylpropylamino) benzoic acid; Bcl-2, B-cell lymphoma-2; BAX, Bcl-2-associated X.
