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Effect of NPPB on intracellular ROS production in HLECs. HLECs were treated with 50 µM NPPB for 24 h. ROS were stained with a dichloro-dihydro-fluorescein diacetate fluorescent probe and examined using ImageJ software. Scale bar, 50 µm. The results are presented as the mean ± standard deviation (n=3). **P<0.01. HLEC, human lens epithelial cell; NPPB, 5-nitro-2-(3-phenylpropylamino) benzoic acid; ROS, reactive oxygen species.
