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. 2021 Feb 22;16:1473–1485. doi: 10.2147/IJN.S284518

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© 2021 Liu et al.

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Characterization of nanomaterials. (A) TEM image of PHSA-ICG and PHSA-ICG-TAT negatively stained with phosphotungstic acid. (B) The average particle size of PHSA-ICG and PHSA-ICG-TAT. (C) UV−vis spectra of PHSA, PHSA-ICG, and PHSA-ICG-TAT (equivalent to 5 μM ICG), and ICG in PBS; the inset shows images of the nanomaterials. (D) The dispersion stability of PHSA-ICG and PHSA-ICG-TAT (equivalent to 10 μM ICG) in PBS, RPMI 1640 medium, and FBS. (E) The uptake capacity of 4T1 cells for ICG, PHSA-ICG, and PHSA-ICG-TAT (equivalent to 10 μM ICG).

Abbreviations: FBS, fetal bovine serum; ICG, indocyanine green; PBS, phosphate-buffered saline; PHSA, PEGylated human serum albumin; TEM, transmission electron microscope; UV, ultraviolet.