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. 2021 Feb 22;17:613–626. doi: 10.2147/NDT.S289444

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© 2021 Zhan et al.

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Berberine regulated CUMS mice depressive behavior and hippocampal neurons growth by targeting miR-34b-5p. (A–D) CUMS mice were divided into 5 groups, including control, CUMS, CUMS + Ad-miR-NC, CUMS + Ad-miR-34b-5p, CUMS + Ad-miR-34b-5p + Berberine. (A) QRT-PCR was used to test miR-34b-5p expression. The sucrose preference, swimming time and immobility time of mice were determined using SPT (B) and FST (C and D). (E–G) Hippocampal neurons were transfected with miR-34b-5p mimic or miR-NC, or treated with Berberine. Non-transfected and non-treated cells were used as control. (E) CCK8 assay was used to detect cell viability. The apoptosis rate and TUNEL positive cells were determined using flow cytometry (F) and TUNEL assay (G) to assess cell apoptosis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.