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. 2021 Feb 22;17:613–626. doi: 10.2147/NDT.S289444

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© 2021 Zhan et al.

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MiR-34b-5p and miR-470-5p directly interacted with BDNF. (A) The sequences of WT/MUT-BDNF 3ʹUTR-1 and WT/MUT-BDNF 3ʹUTR-2 were shown. Dual-luciferase reporter assay (B) and RIP assay (C) were used to evaluate the interaction between BDNF and miR-34b-5p or miR-470-5p. (D) The transfection efficiencies of miR-34b-5p mimic and inhibitor were evaluated by measuring miR-34b-5p expression in hippocampal neurons. (E) MiR-470-5p expression was determined using qRT-PCR to assess the transfection efficiencies of miR-470-5p mimic or inhibitor in hippocampal neurons. (F and G) BDNF protein expression was tested by WB analysis in hippocampal neurons transfected with miR-34b-5p and miR-470-5p mimics or inhibitors. **P < 0.01, ***P < 0.001, ****P < 0.0001.