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. 2021 Feb 22;17:613–626. doi: 10.2147/NDT.S289444

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© 2021 Zhan et al.

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Berberine mediated BDNF expression to regulate CUMS mice depressive behavior and hippocampal neurons growth. (A–D) C57BL/6J mice were divided into 5 groups, including control, CUMS, CUMS + sg-NC, CUMS + sg-BDNF, CUMS + sg-BDNF + Berberine. (A) BDNF protein expression was determined using WB analysis. SPT (B) and FST (C–D) were used to evaluate the sucrose preference, swimming time and immobility time of mice. (E–G) Hippocampal neurons were transfected with si-NC or si-BDNF, or treated with Berberine. Non-transfected and non-treated cells were used as control. (E) The viability of cells was tested using CCK8 assay. Flow cytometry (F) and TUNEL assay (G) were employed to detect cell apoptosis rate and TUNEL positive cells to assess cell apoptosis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.