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. 2021 Feb 22;13:1803–1815. doi: 10.2147/CMAR.S276290

Figure 1.

Figure 1

Loss of LINC00504 restricts the malignant behavior of breast cancer cells in vitro. (A) LINC00504 expression in breast cancer was analyzed using the TCGA and GTEx databases. (B) qRT-PCR was used to measure LINC00504 expression in 57 pairs of breast cancer tissues and corresponding adjacent normal tissues. (C) The expression of LINC00504 in five breast cancer cell lines (BT-474, MCF-7, MDA-MB-231, SKBR3 and T-47D) and in a human immortalized breast epithelial cell line (MCF-10A). (D and E) The correlation between LINC00504 expression and tumor stage or overall survival in breast cancer was analyzed using the TCGA and GTEx databases. (F) qRT-PCR was done to assess the transfection efficiency of si-LINC00504 in MCF-7 and MDA-MB-231 cells. (G) CCK-8 assay was applied to measure the proliferation of MCF-7 and MDA-MB-231 cells after LINC00504 silencing. (H) Apoptosis of si-LINC00504- or si-NC-transfected MCF-7 and MDA-MB-231 cells was examined by flow cytometry. (I and J) Transwell experiments were employed to measure the migration and invasion capacities of MCF-7 and MDA-MB-231 cells after si-LINC00504 or si-NC transfection. **P < 0.01.