Fig. 2.

Midbrain microglia and astrocyte markers and microglial and astrocytic cell density in schizophrenia (SCZ) and in adult mice prenatally exposed to the viral mimetic, poly(I:C). a Representative immunohistochemical staining showing microglial cells in the human midbrain, which were identified by brown HLA+ staining in the cytoplasm and processes. Nuclei are stained blue with a nuclear dye (Nissl). b HLA-DR+ cell number in midbrain from SCZ cases compared with control cases. c HLA-DR, d AIF1, e CD68 f TSPO, and g GFAP mRNA levels in midbrain from SCZ cases compared with control cases. Representative immunohistochemical staining of h AIF1+ and m CD68+ microglial cells and q GFAP+ astrocytes in mouse midbrain. i AIF1+ and n CD68+ microglial cell density and r GFAP+ astrocyte cell density in midbrain from adult mice prenatally exposed to poly(I:C) or vehicle control. AIF+ microglial cell j soma area, k primary processes, and l process branch points in midbrain from adult mice prenatally exposed to poly(I:C) or vehicle control. o AIF1 p TSPO, and s GFAP mRNA levels in midbrain from adult mice prenatally exposed to poly(I:C) or vehicle control. Data are mean ± SEM. Scale bars = 20 μm