Fig. 6.

Tcf4−, Tbr1−, and Top3b-associated molecular pathways. a Proteomics analysis was used to identify dysregulated proteins owing to Tcf4, Tbr1, or Top3b knockdown. Dysregulated proteins were analyzed in STRING to identify PPI networks. Significant PPI enrichment was only detected in the Tbr1 knockdown protein set. Red, blue, and green circles are query proteins that are dysregulated upon Tcf4, Tbr1, or Top3b knockdown, respectively, gray circles are candidate proteins added by STRING. b The same analysis was performed for the combined dysregulated protein set, identifying the SNARE complex as a potential common target of Tcf4, Tbr1, and Top3b. c GO enrichment analysis of the combined dysregulated protein set (without addition of candidate interactors) confirms enrichment for proteins involved in “SNARE binding” and “syntaxin binding” contributed by TCF4−, TBR1−, and TOP3B-dysregulated proteins