Fig. 4.

a Volcano plot visualising unsupervised proteomics results (normalised by log2 transformation followed by median subtraction in Perseus, followed by Student’s t test with permutation based FDR calculation, n = 6, age WT 114 ± 1 days/A178D 113 ± 1 days, 3M/3F). Significantly downregulated proteins are telethonin and Fhl2, significantly upregulated proteins are αβ-crystallin (αβ-cryst), heat shock protein 27 (Hsp27), Dnaj homology subfamily B member (Dnajb6, another heat shock protein) and myeloid leukaemia factor 1 (Mlf1), all shown in red. b Representative Western blot demonstrating the downregulation of Fhl2 in total extracts of A178D mice. Gapdh serves as loading control. c Quantification of blot in panel B indicates downregulation of Fhl2 by approximately 50%; (mean ± SEM, n = 6, age WT 114 ± 1 days/A178D 113 ± 1 days, 3M/3F, Student’s t test)