Fig. 6.

CRISPR-Cas9 genome editing was performed on IPF fibroblasts targeting the Smad3 and TGM2 gene. IPF fibroblasts underwent CRISPR-Cas9 genome editing as described in the “Materials and methods” targeting the SMAD3 and TGM2 genes. Representative images of immunocytochemistry staining of TG2 in the TG2 KO, SMAD3 KO and WT IPF fibroblasts in the absence a and presence c of TGFβ1 stimulation. TG2 (green), Hoechst (blue). b and d Graphs present the percentage of the signals measured via immunocytochemistryas described in the “Materials and methods”. Quantitative data was generated using the ‘Mean_TargetAvgIntenCh2′ algorithm and the ‘AP30704v1_SIAJCollagenandCellCountCellHealth (V01)’ protocol. Data are expressed as a percentage of control ± SEM. **, p < 0.01; and ***, p < 0.001