Fig. 1.

Comparison of fluorescence preservation in lungs of Club-cell specific reporter mice. a Schematic representation of the experimental procedure. To achieve tissue transparency, lungs were dehydrated using different pH-adjusted alcohol series and cleared in the organic solvent ethyl cinnamate (ECi). b–e 3D reconstructions of lung lobes after dehydration/clearing in ethanol/ECi (b), tert-butanol/ECi (c), 1-butanol/ECi (d) and 1-propanol/ECi (e). To evaluate the degree of auto- vs. epi-fluorescence, wildtype (wt) and transgenic (tg = Scgb1a1-mCherry) lung lobes were recorded simultaneously by light sheet microscopy. Scale bar: 500 µm