Fig. 2.

Visualization of Club cell depletion and recovery in whole lungs following experimental injury. a Schematic representation of the experimental procedure. To induce bronchiolar damage, Scgb1a1-mCherry animals were challenged by single intraperitoneal injection of naphthalene (200 mg/kg). Lungs were harvested at different days post naphthalene (dpn). b–i 3D reconstructions of whole lung preparations (b, d, f, h; scale bar: 2 mm) and magnified areas (c, e, g, i; scale bar: 200 µm) isolated from control (b-c) and injured Scgb1a1-mCherry animals at 3 dpn (d–e), 7 dpn (f–g) and 21 dpn (h–i). Dotted lines depict bronchiolar boundaries, asterisks mark air inclusions with intrinsic background fluorescence