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. 2021 Feb 26;11:4759. doi: 10.1038/s41598-021-84237-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cytopathic effects in primary porcine alveolar macrophages (PAM) and immortalized porcine kidney macrophages (IPKM). Cell cultures were mock-inoculated or inoculated with Armenia07, Kenya05/Tk-1 and Espana75 isolates (MOI = 0.1). The left and right columns show the PAM and IPKM cell lines at 3 days after inoculation, respectively. Images are representatives from three experiments that demonstrated similar trends. Bar = 50 µm.