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. 2021 Feb 26;11:4759. doi: 10.1038/s41598-021-84237-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the African swine fever virus (ASFV) production in primary porcine alveolar macrophages (PAM) and immortalized porcine kidney macrophages (IPKM). Cell cultures were infected with Armina07 (a), Kenya05/Tk-1 (b), Espana75 (c) and Lisobon60V isolates (d) (MOI = 0.001). The culture supernatant was recovered at the indicated time-point infection. Viral production in the PAM and IPKM cell cultures were estimated by titration. Data represent the means and standard deviations of three experiments. Asterisks indicate statistically significant differences in viral production in the PAM and IPKM cell cultures (p < 0.05, Student’s t-test).