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. 2021 Feb 26;11:4737. doi: 10.1038/s41598-021-81714-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HSA is an essential component for the differential expression of genes in A. baumannii. A. baumannii A118 cells were cultured in LB or LB supplemented with one of the following: 4% CSF, HSA-depleted CSF (dCSF), or dCSF + 0.2% HSA and its cDNA was synthesized. RT-qPCR was conducted with three genes (A) fimA encoding gene, (B) vgrG1, and (C) bauD. Shown are the means of the results obtained from three independent experiments. The y axis refers to the fold difference of each gene to the threshold cycle (C_T) values corresponding to recA; the standard deviation SD is shown. Asterisks indicate significant differences among treatments, as determined by ANOVA followed by Tukey’s multiple comparison test (P < 0.05).