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. 2021 Feb 24;40(1):21–27. doi: 10.1089/mab.2020.0033

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© Natsuko Mizutani et al., 2021; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — Comparison between JMAM-1 and other mAbs by XTT assay. NCI-H226 cells (3 × 10³ cells/well) were seeded in a 96-well plate and XTT assay was performed. JMAM-1 concentrations used for dose–response analyses were 0.001, 0.005, 0.025, 0.1, 0.4, and 2 μM. After 48 hours, ERC-mesothelin and anti-CD26 were prepared using the same concentrations and absorbance was read at 450 nm using an ELISA plate reader (Bio Tek Instruments, Northern Vermont, USA). Data were analyzed using Prism 5 statistical software (n = 3; p < 0.05) through one-way analysis of variance, followed by Tukey test. JMAM-1 mAb showed almost the same cell growth inhibitory effects as anti-CD26 and mesothelin.