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Apparent hydrodynamic radius of TNF-α-alexa488 (100 nM) as function of adalimumab concentration (0 – 1000 nM) determined by FIDA in 67 mM phosphate buffer with 0.1% v/v HSA at pH 7.40 and 25 °C using pre-incubated samples (n = 3, error bars represent standard deviation). The solid line shows fitting to the excess indicator binding isotherm [Eq. (7)].
