Fig. 5. The effects of paraformaldehyde killed OP50 on fecundity, development, and lifespan.

a Average progeny number of worms fed with different bacteria conditions. b Development of worms sorted based on relative axial length (Time of flight, TOF) at L4. c Development time (hours) of worms from egg to egg laying on different conditions of OP50. d Images of worms on various food sources at young adult (YA), day 2, and day 4 adult. e Area of the worms (µm²) quantified from images in Fig. 5d. f Percent alive of worms fed with treated and control OP50. Single asterisk denotes p-value < 0.05, double asterisks denote p-value < 0.005, triple asterisks denote p-value < 0.0005, and quadruple asterisks denote p-value < 0.0001.