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. 2021 Feb 26;12:1333. doi: 10.1038/s41467-021-21594-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Volcano map depicting genes upregulated (red) or downregulated (blue) 2-fold or more in T_FH cells on 8 dpi. Heatmap of differentially expressed genes is shown on the right. b Gene Ontology (GO) terms of the differentially expressed genes in Mettl3^fl/flCd4-Cre T_FH cells compared with Ctrl T_FH cells (Upregulated: top panel; Downregulated: bottom panel). c Gene set enrichment analysis (GSEA) of T_FH and GC T_FH gene set in Mettl3^fl/flCd4-Cre T_FH cells relative to expression in Ctrl T_FH cells as in a. d GSEA analysis of “TCF-1-activated genes in T_FH cells” and “TCF-1-suppressed genes in T_FH cells” (GSE65693) in Mettl3^fl/flCd4-Cre T_FH cells relative to expression in Ctrl T_FH cells as in a. Heatmaps representation of top 30 ranking genes in the leading edge are shown, respectively. e Quantitative RT-PCR analysis of selected interested genes in a, relative expression was normalized to that in Ctrl T_FH cells (n = 7 for Ctrl group, n = 8 for Mettl3^fl/flCd4-Cre group except Bcl6 gene (n = 7 per group)). Data are from one experiment with triplicates (a–d) or pooled from two experiments (e). Error bars indicate standard error of the mean. P value was calculated by unpaired two-tailed Student’s t test (e).