Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 26;12(2):217. doi: 10.1038/s41419-021-03508-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Autophagy activation or inhibition by pretreatment with Rapa or 3-MA, respectively. The samples were collected 12 h after CLP-induced SAKI. A, B The number of autophagosomes in renal epithelial cells was calculated in 20 randomly selected fields using a transmission electron microscope (black arrow: autophagosomes; Mito: mitochondrion; upper panel: magnified ×8000 and scale bar = 2 μm; lower panel: magnified ×20000 and scale bar = 1 μm, n = 20). C The irregular brush border (arrow) and ectasia of affected tubules (arrowheads) were observed by H&E staining (upper panel, 200×; inset: 400×; scale bar = 10 μm) and PAS staining (lower panel, 200×; inset: 400×; scale bar = 10 μm) of the kidney cortex. D The tubular damage score was evaluated based on H&E and PAS staining (n = 10). E Scr levels (n = 6). F Serum BUN levels (n = 6). The data are presented as the mean ± SD. ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs the sham group; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 vs the vehicle + CLP group. RTECs renal tubular epithelial cells, SAKI sepsis-induced acute kidney injury, CLP caecal ligation and puncture, PAS periodic acid-Schiff staining, H&E haematoxylin–eosin staining, Rapa rapamycin, Scr serum creatinine, BUN urea nitrogen.