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. 2021 Feb 26;11:4735. doi: 10.1038/s41598-021-84023-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative and qualitative assessment of PDCA-1⁺ cells in the spinal cord. (a) Flow cytometry analysis of host GFP⁻/CD45.2 PDCA-1⁺ cell counts at d-18 in the spinal cord of control mice with EAE injected with PBS (n = 8) relative to those in CpG-pre-pDC (n = 13) and PBS-pre-pDC (n = 5) recipients. Data from two cumulated experiments. No significant differences were found using one-way ANOVA with Bonferroni post-test. (b, c) Cytokine expression measured using isotype controls, after 4 h activation with PMA/ionomycin in presence of brefeldin, by host GFP⁻PDCA-1⁺ cells at d-18 in the spinal cord of the same three groups of mice (n = 4 controls, n = 3 CpG-pre-pDC recipients and n = 3 PBS-pre-pDC recipients). Shown are representative FACS profiles (b) and histograms of percentages expressed as mean ± s.e.m. (c) No significant statistical differences were found using one-way ANOVA with Bonferroni post-test. (d, e) Compared cytokine expression analysis at d-15 in CD45.1 progenitor-derived and CD45.2 host-derived PDCA-1⁺ cells of the spinal cord of CpG-pre-pDC recipients. Shown are representative FACS profiles (d) and histograms of percentages (e) expressed as mean ± s.e.m. (n = 5 mice/group). No significant differences using Students’ t-test. (f, g) Compared cytokine expression analysis at d-15 in host CD45.2 PDCA-1⁺ cells in control mice with EAE injected with PBS and in CpG-pre-pDC recipients. Shown are representative FACS profiles (f) and histograms of percentages (g) expressed as mean ± s.e.m. (n = 5 mice). Statistical analysis using Students’ t-test, *, p = 0.0291.