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. 2021 Feb 26;12:1167. doi: 10.1038/s41467-021-21180-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — After building the reference pan-genome, CORRECT mode calculates fragment weights and lab weights according to the pan-genome annotations. To predict the lab-of-origin of an unknown sequence, PlasmidHawk first aligns the query sequence to the pan-genome and identifies candidate source labs through MAX mode: in this case, lab A and lab C. CORRECT mode then calculates lab scores for the labs output from MAX mode. In the end, CORRECT mode predicts the lab with the minimum lab score, lab A, as the depositing lab.