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. 2021 Feb 26;10(2):17. doi: 10.1038/s41389-021-00307-0

Fig. 6. JMJD3 promotes TGF-β-induced EMT by increasing syntenin-mediated formation of TβRI/Smad2/3 complex.

Fig. 6

a Expression of TβRI and TβRII protein expression in BZR cells transfected with Flag-TβRI and/or syntenin shRNA. b Expression of TβRI and TβRII protein expression in BZR cells transfected with Flag-TβRI and/or JMJD3 shRNA. c Immunofluorescence analysis of expression and localization of Flag-TβRI (green) in A549 cells in response to knockdown of JMJD3. DAPI-staining nuclei (blue) were also shown. Scale bar, 20 μm. d Immunoprecipitates (IP) analysis of protein interactions between TβRI and smad2 in A549 cells transfected with the increasing amounts of HA-tagged JMJD3 expression vector (1 and 3 µg) in response to TGF-β1 (5 ng/ml) for 1 h. e IP analysis of protein interactions between TβRI and smad2 in BZR cells transfected with Flag-syntenin expression vector in response to TGF-β1 (5 ng/ml) for 1 h. f IP analysis of protein interactions between TβRI and smad2 in Flag-TβR1-expressed A549 cells treated with Salirasib (10 μM) for 24 h in response to either and TGF-β1 (5 ng/ml) for 1 h.