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. 2021 Feb 26;4:255. doi: 10.1038/s42003-021-01774-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Normalized (to CD31) expression of EC adhesion molecules associated with endothelial activation (n = 3 devices). B Normalized (to β-actin) expression of ICAM-1 on ECs (HUVEC) and stromal cells (nHLF) cultured in well plates after treatment with hyaluronidase (n = 3 wells). C Imaging of ICAM-1 after MVN treatment. The scale bar is 200 µm. D ICAM-1 is not highly expressed on ECs in the vicinity of TCs arrested for 6 h prior to fixing (white arrow), as compared to ICAM-1 expressed on stromal cells (red arrows). The scale bar is 60 µm. Statistical significance was assessed by student’s t test assuming normally distributed data, p < 0.05 *, p < 0.01 **, p < 0.001 ***.