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. 2021 Feb 26;7:38. doi: 10.1038/s41420-021-00421-3

Fig. 4. Cholesterol alleviates the inhibitory effect of LRP6 on FZD7-PCP pathway to promote YAP expression in colon cancer cells.

Fig. 4

A SW1116 and SW480 were transfected with negative control siRNA or FZD7 siRNA for 72 h, respectively. B SW1116 and SW480 were transfected with negative control siRNA or FZD7 siRNA for 48 h, and starved in serum-free medium for 24 h, then incubated with DMSO or cholesterol (5 μM) for 2 h, respectively. C SW1116 cells were starved for 24 h, then incubated with DMSO or cholesterol (5 μM) for 2 h. RhoA activity was detected by GST pull-down assay. D SW1116 and SW480 were transfected with negative control siRNA or RhoA siRNA for 48 h, then starved for 24 h, and incubated with DMSO or cholesterol (5 μM) for 2 h, respectively. E SW1116 were transfected with siLRP6, siLRP6#1, and siLRP6#UTR (targeting 5’-untranslated region of LRP6 mRNA) for 72 h. F Schematic diagram of LRP6 and LRP6 deletion mutants. G SW1116 stably expressing shLRP6#UTR was constructed by lentiviral vector. SW1116 stable cell line were transfected with control vector or LRP6 and deletion mutants vectors for 72 h. H SW1116 was transfected with negative control siRNA or siLRP6, in the presence or absence of FZD7 deletion. I SW1116 was transfected with vectors expressing LRP6 and FZD7 for 48 h, followed by starvation for 24 h, and then treated with cholesterol (5 μM) or DMSO for 2 h. Co-IP assay was performed to detect the interaction between LRP6 and FZD7.