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Metabolic labeling and isolation of SA-presenting microbes from a fecal microbiome sample. Fecal samples collected from a healthy human donor were cultured with the metabolic probe N-acetyl-9-azido-9-deoxy-neuraminic acid (Neu5Ac9N3/Sia9N3). The Sia9N3 incorporating bacterial cells were labeled with biotin through copper-free “click” reaction and were detected and isolated with flow cytometry.
